What’s Different from bwa-mem2

This section tracks every change that bwa-mem3 carries on top of upstream bwa-mem2/bwa-mem2’s master branch, explains why each change was made, and records its upstream disposition.

How this section is organized

Each deep-dive page covers one category of change:

• Correctness fixes — bugs in upstream bwa-mem2 that are fixed in bwa-mem3, including the kswv SIMD score2 plateau series, the proper-pair flag regression, the zero-init crash, the SMEM buffer overflow, and the @PG tab-escape issue.
• Performance improvements — lockstep SMEM batching, batched -H header ingestion, libsais FM-index construction, and the consolidated mapping speedup suite.
• Features — --meth bisulfite mode, mimalloc allocator, --supp-rep-hard-cap, bwa-mem3 shm, shm --meth, the HN:i tag, and the --bam=LEVEL output flag.
• Architecture support — the Linux ARM64/aarch64 build, the arch=avx512bw Makefile target, the NEON kswv mate-rescue kernel, and the AVX2 kswv mate-rescue kernel.
• Build & infrastructure — the doctest framework, Codecov integration, PACKAGE_VERSION from git describe, PGO target parameterization, CXXFLAGS/CPPFLAGS/LDFLAGS forwarding, the unit-test harness, and the CI matrix expansion.
• Upstream PR status — a single table cross-referencing every fork-carried change to its corresponding upstream PR or issue, with current upstream disposition.

Carried on top of upstream

Auto-generated from git log --reverse --no-merges master..main and the conventional-commits scope on each PR-merge title; do not edit by hand. For per-PR upstream disposition (bwa-mem2 PR / issue refs and status), see Upstream PR status.

Additional fork-level changes

• Vendored mimalloc allocator: ext/mimalloc is pinned at v3.3.0 and linked into every binary by default (USE_MIMALLOC=1). Linux uses --whole-archive static linkage; macOS uses dyld-interposed shared linkage. USE_MIMALLOC=1 is the supported and recommended default on all platforms; USE_MIMALLOC=0 is provided as a best-effort opt-out and is CI-gated on Linux x86 only. See Features for details.

• --supp-rep-hard-cap INT (opt-in, default disabled): forces MAPQ=0 on supplementary alignments whose chain contains a seed with >=INT genome occurrences. Addresses the long-standing bwa/bwa-mem2 issue where a supp fragment that maps to many places standalone (e.g. a short read in a CCATCC repeat) inherits a high MAPQ from its primary because the supp’s competing repetitive chains get filtered out during the full-read pipeline and therefore never contribute to its sub/sub_n. See upstream #260 for the reporter case. Primary MAPQ is unaffected; default output is byte-identical to stock bwa-mem2. Typical values are 5–20 (lower = more aggressive); the upstream #260 repro drops from MAPQ=60 to MAPQ=0 at --supp-rep-hard-cap 18.

Version stamping

PACKAGE_VERSION (the value reported by bwa-mem3 version and written to the @PG VN: SAM header field) is generated at build time by the Makefile from git describe --tags --dirty, e.g. v2.3-30-g61813ef for a tree 30 commits past upstream tag v2.3 at commit 61813ef.

• No manual bumping required: cut a fresh release by tagging the commit (git tag -a vX.Y-fg-labs.N -m ...) and the next build picks it up.
• Builds where git describe --tags fails (source-tarball extractions, or shallow clones / checkouts with no tag reachable from HEAD — including CI’s default actions/checkout fetch-depth of 1) fall back to the static FG_LABS_VERSION_FALLBACK in Makefile. Bump that when cutting a release that will be consumed as a tarball, or in CI artifacts.
• src/version.h is generated and .gitignored; make clean removes it.

Branching and update policy

• master tracks upstream unchanged.
• main is upstream/master plus the commits above. Rebased onto upstream roughly quarterly, or sooner when an upstream release we care about lands.
• Contributions go via PR targeting main. CI and CodeRabbit gate merges.
• Any PR that adds or removes a fork-carried commit must update the table above in the same PR.

Consuming

Clone this repo and check out main:

git clone https://github.com/fg-labs/bwa-mem3.git
cd bwa-mem3
git checkout main

Or vendor the branch into a downstream repo by pinning to a specific commit (not the branch tip) so your build is reproducible.

Relationship to upstream

We submit the generally-useful fixes and features carried here as PRs against bwa-mem2/bwa-mem2 when the upstream maintainers are actively merging; while they are not, fixes land here first and we drop them from main once they appear upstream.

See also: Correctness fixes · Performance improvements · Features · Upstream PR status · Developer Guide → Contributing